Full Time 40 hours Grade 077 Pediatrics Med Ctr Schedule 8 AM-4:30 PM OR 9AM-5:30PM; SCHEDULE WILL VARY BASED ON OPERATIONAL NEEDS; EVENINGS, WKENDS & HOL POSSIBLE; Responsibilities Position Summary: This position requires active participation in and provides technical support for research activities performed in a Cancer Biology Laboratory. Projects in the laboratory focus on understanding factors involved in tumorigenesis and therapeutic resistance with an overall goal of developing small molecule compounds for targeted cancer therapy. This individual will participate in basic science laboratory procedures including routine animal experiments as well as technical functions including the development of laboratory safety/experimental protocols, and maintenance of laboratory supplies and equipment. Research will require prior experience with mouse breeding/genotyping, tissue culture procedures, and immunohistochemistry. This position will involve handling mice and subcutaneous/intraperitoneal mouse injections. The technician must be able to work in a collaborative environment and must be comfortable working with mice. Results from these experiments will be used in both publications and grant proposals. Responsibilities Animal Procedures Perform daily tasks associated with the care, breeding and treatment of research mice for experimental purposes. Manage genetically engineered mouse model colony. Perform animal identification, mating, tail clipping, genotyping, weaning, euthanasia of undesirable genotypes, and overall colony management. Observe and record physical signs of animal health. Perform tumor implantation subcutaneously with cancer cell lines or patient-derived tumor cells. Dose animals via intraperitoneal injection and measure tumor response via caliper and non-invasive imaging methods. Perform non-survival animal surgeries for collection of tumor specimens. Generate RNA and protein from tumor samples for molecular characterization, including immunohistochemistry, Western blots and RT-qPCR. Duties and procedures may include, but are not limited to: receipt, restraint, and handling of laboratory animals; performance of physical exams, surgical procedures, and treatments; tumor sample collection and characterization; and disposal of biologic specimens, euthanasia, and necropsy of experimental animals. Recording of pertinent data in study records: maintain/update accurate, detailed records of genotyped mice, experimental data and protocols in accordance with methods requested. Research Laboratory Procedures Isolate primary tumor cells from mouse tumors using basic tissue culture procedures. Culture human/mouse cancer cell lines, including monitoring of cultures using trypan counts. Performing routine laboratory analyses including (but not limited to) RT-qPCR, Western blot, Immunohistochemistry, and microscopy. General Monitor and ensure training and compliance with Environmental Health and Safety (EHS). Ensure that health and safety practices are maintained in accordance with University policy and the law. Record laboratory and other training as required.Oversee regulatory issues including biosafety, environmental health and radiation usage.Responsible to implement changes, respond to issues related to but not limited to laboratory inspections conducted by EHS, UCAR, and IBC. Contribute to and manage UCAR and IBC protocols. Organize work stations with necessary supplies, including assembling any necessary plastic ware, preparing general buffers and restocking of work station supplies. Oversee routine maintenance/cleaning, management, and/or repair of instrumentation per protocol. Independently manage the laboratory's chemical inventory. Monitor and coordinate ordering of lab supplies ensuring orders as properly processed. Perform in depth comparative research to obtain the best product at the best price and provide informed recommendations to the PI for decision making. Help to train and assist new laboratory personnel and students in terms of execution and troubleshooting of their experimental work including research compliance with regulations and general laboratory research techniques. Orient new and collaborating laboratory personnel to laboratory-related procedures. Participate in laboratory meetings and relevant seminars pertinent to the research goals of the laboratory. Keep current in the field through literature. Meet with PI on a regular basis to discuss research findings and make recommendations regarding future directions. Requirements: Associateâ™s degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. BS/BA in biological/physical science or equivalent experience is preferred. Advanced education and experience is valued. A minimum of 1-year experience in laboratory science desired. Experience working with mice/rodents is required. Prior experience performing subcutaneous and intraperitoneal mouse injections is preferred. Prior experience and/or courses in molecular biology are a plus. Candidate should be highly organized and detail/documentation oriented. Attention to detail and meticulous ability to maintain records is essential. Candidate must be able to work in a collaborative work environment. Strong interpersonal skills, including verbal and written communication are essential. Advanced computer skills including MS Word and Excel, are required. Experience using statistical software packages such as Prism is preferred. Occasional evening or weekend hours may be necessary. Schedule varies based on operational needs. Knowledge of University policy and procedures is desirable. Must have reliable/efficient transportation. Knowledge of general laboratory safety processes, universal precautions, aseptic cell culture technique, handling of mice and practice of rodent euthanasia is required. Thorough documentation of results in written and electronic database formats will be required. Skills in basic database management and generation of publication-quality figures are preferred.
Internal Number: 1151119226
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